Categories
Use: sufficient for 250 colorimetric tests
Detection method: colorimetric
Storage temperature: −20 ° C
General description
Magnesium, one of the most abundant trace metals in living organisms, contributes to a variety of biological functions, including ATP and nucleic acid processing, energy metabolism, and enzyme function. Magnesium forms complexes with multiple molecules such as phospholipids and ATP. Low serum magnesium levels have been associated with metabolic syndrome, type 2 diabetes mellitus, and hypertension, as well as increased smooth muscle and platelet reactivity.
Request
The Magnesium Assay Kit has been used to quantify magnesium levels in conditioned media.
Suitability
Suitable for quantifying magnesium concentrations in a variety of biological samples.
Beginning
The Magnesium Assay Kit provides a simple and straightforward procedure for measuring magnesium in a variety of samples. Magnesium concentration is determined by a coupled enzyme assay that takes advantage of the specific glycerol kinase requirement for Mg2 +, resulting in a colorimetric product (450 nm) proportional to the magnesium present. This assay gives a linear range of 3-15 moles of magnesium and shows no detectable interference with Fe2 +, Cu2 +, Ni2 +, Zn2 +, Co2 +, Ca2 +, and Mn2 +.
