Categories
Use: enough for 200 tests
Quality level: 200
Detection method: colorimetric
Sent in: wet ice
Storage temperature: 2-8 ° C
General description
Free radicals or reactive oxygen species (ROS) are produced during biochemical redox reactions as part of normal physiological cellular metabolism (protection against infectious organisms) and in response to environmental factors such as ultraviolet light, cigarette smoke, environmental pollutants, and radiation.
Once formed, ROS attack cellular components and damage lipids, proteins, and DNA, which can trigger numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders. In the presence of antioxidants, the radical cation is suppressed to a degree that depends on the activity of the antioxidant and the intensity of the color decreases proportionally. Trolox, a water-soluble vitamin E analog, serves as a standard or control antioxidant.
Request
The Antioxidant Assay Kit provides all the reagents necessary for the effective measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates. It has also been used to measure the cation radical uptake capacity (2,2′-azino-di-3-ethylbenzthiazoline sulfonate) (ABTS +). It was tested on A431 and CHO cell lysates; rat brain, liver, and kidney lysates; human plasma, serum, urine, and saliva.
Suitability
Suitable for measuring the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates.
Beginning
The principle of the antioxidant assay is the formation of ferryl myoglobin radical form metmyoglobin and hydrogen peroxide, which oxidizes ABTS (2,2 ¢ -azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) to produce a radical cation, ABTS · +, a soluble chromogen that is green in color and can be determined spectrophotometrically at 405 nm.
Preparation note
The reagents must be prepared with ultrapure water.
Reconstitution
- Myoglobin solutions should be stored at -20 ° C after reconstitution.
- The Trolox working solution should be stored at -20 ° C after reconstitution.
- The ABTS Substrate Solution should be stored at 4 ° C for 2 weeks or at -20 ° C for 1 month.
