Abstract
Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker it strongly recognises essentially the most cancers specific T antigen (Galbeta1–>3GalNAc-), nevertheless not its sialylated mannequin. Nonetheless, an additional specificity within the course of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.
For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity. Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.
Arachis hypogaea (Peanut) Lectin (PNA) – HRP
Arachis hypogaea lectin (PNA) |
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05-0116-10 | Medicago | 10 mg | EUR 139.2 |
Arachis hypogaea lectin (PNA |
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05-0116-1000 | Medicago | 1 g | Ask for price |
Arachis hypogaea lectin (PNA |
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05-0116-50 | Medicago | 50 mg | EUR 376.8 |
rAra h 1 (Arachis hypogaea 1.0101) |
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10-P202-C100 | ExBio | 0.1mg | EUR 242 |
rAra h 2 (Arachis hypogaea 2.0201) |
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10-P208-C100 | ExBio | 0.1mg | EUR 242 |
rAra h 8 (Arachis hypogaea 8.0101) |
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10-P210-C100 | ExBio | 0.1mg | EUR 242 |
rAra h 9 (Arachis hypogaea 9.0101) |
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10-P232-C100 | ExBio | 0.1mg | EUR 242 |
Arachis hypogaea Arachin Ahy-3 |
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1-CSB-YP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast |
Arachis hypogaea Arachin Ahy-3 |
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1-CSB-EP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli |
Recombinant Arachis hypogaea Conglutin |
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CSB-EP720709ANE | Cusabio | 3989 mg | Ask for price |
Recombinant Arachis hypogaea Conglutin |
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AP73786 | SAB | 1mg | EUR 2826 |
Arachis hypogaea Lectin (PNA) - Cy3 |
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21761088-1 | Glycomatrix | 1 mg | EUR 147.5 |
Arachis hypogaea Lectin (PNA) - Cy3 |
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21761088-2 | Glycomatrix | 2 mg | EUR 200.92 |
Arachis hypogaea Lectin (PNA) - Cy5 |
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21761089-1 | Glycomatrix | 1 mg | EUR 147.5 |
Arachis hypogaea Lectin (PNA) - Cy5 |
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21761089-2 | Glycomatrix | 2 mg | EUR 258.83 |
Arachis hypogaea Lectin (PNA) - HRP (Horseradish Peroxidase) |
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21510927-1 | Glycomatrix | 2 mg | EUR 136.34 |
Arachis hypogaea Lectin (PNA) - Pure |
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21510000-1 | Glycomatrix | 5 mg | EUR 81.38 |
Arachis hypogaea Lectin (PNA) - Pure |
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21510000-2 | Glycomatrix | 10 mg | EUR 128.76 |
Arachis hypogaea Lectin (PNA) - Pure |
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21510000-3 | Glycomatrix | 50 mg | EUR 335.68 |
Arachis hypogaea Lectin (PNA) - Ferritin |
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21510500-1 | Glycomatrix | 1 mg | EUR 145.5 |
Recombinant Arachis hypogaea Conglutin-7 |
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CSB-EP757666ANE | Cusabio | 4099 mg | Ask for price |
Recombinant Arachis hypogaea Conglutin-7 |
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AP71363 | SAB | 1mg | EUR 2826 |