
Abstract
Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker it strongly recognises essentially the most cancers specific T antigen (Galbeta1–>3GalNAc-), nevertheless not its sialylated mannequin. Nonetheless, an additional specificity within the course of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.
For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity. Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.

Arachis hypogaea (Peanut) Lectin (PNA) – HRP
Arachis hypogaea lectin (PNA) |
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05-0116-10 | Medicago | 10 mg | EUR 139.2 |
Arachis hypogaea lectin (PNA |
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05-0116-1000 | Medicago | 1 g | Ask for price |
Arachis hypogaea lectin (PNA |
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05-0116-50 | Medicago | 50 mg | EUR 376.8 |
Arachis hypogaea Arachin Ahy-3 |
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1-CSB-EP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli |
Arachis hypogaea Arachin Ahy-3 |
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1-CSB-YP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-YP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast |
Arachis hypogaea Allergen Ara h 1, clone P41B |
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1-CSB-CF337356ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system |
CF568 Arachis hypogaes Lectin PNA (Peanut) |
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29061 | Biotium | 1mg | EUR 234 |
Description: Minimum order quantity: 1 unit of 1mg |
CF594 Arachis hypogaes Lectin PNA (Peanut) |
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29062 | Biotium | 1mg | EUR 234 |
Description: Minimum order quantity: 1 unit of 1mg |
CF488A Arachis hypogaes Lectin PNA (Peanut) |
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29060 | Biotium | 1mg | EUR 234 |
Description: Minimum order quantity: 1 unit of 1mg |
CF640R Arachis hypogaes Lectin PNA (Peanut) |
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29063 | Biotium | 1mg | EUR 234 |
Description: Minimum order quantity: 1 unit of 1mg |
Arachin Ahy-3 Antibody, HRP conjugated |
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1-CSB-PA737391LB01ANE | Cusabio |
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Description: A polyclonal antibody against Arachin Ahy-3. Recognizes Arachin Ahy-3 from Peanut. This antibody is HRP conjugated. Tested in the following application: ELISA |
Arachin Ahy-3 Polyclonal Antibody, HRP Conjugated |
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A56271 | EpiGentek |
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QPCR Kit DNA Arachis hyp |
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MOL6482 | Scientific Laboratory Supplies | EACH | EUR 1118.9 |
QPCR Kit DNA Arachis hyp Mmix |
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MOL6484 | Scientific Laboratory Supplies | EACH | EUR 3461.15 |
BSA Conjugated Arachidonic Acid (AA) |
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4-CPB098Ge11 | Cloud-Clone |
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Description: Recombinant Pan-species Arachidonic Acid expressed in: Protein conjugation |
OVA Conjugated Arachidonic Acid (AA) |
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4-CPB098Ge21 | Cloud-Clone |
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Description: Recombinant Pan-species Arachidonic Acid expressed in: chemical synthesis |
Arachin Ahy-3 Antibody, FITC conjugated |
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1-CSB-PA737391LC01ANE | Cusabio |
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Description: A polyclonal antibody against Arachin Ahy-3. Recognizes Arachin Ahy-3 from Peanut. This antibody is FITC conjugated. Tested in the following application: ELISA |
Arachin Ahy-3 Antibody, Biotin conjugated |
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1-CSB-PA737391LD01ANE | Cusabio |
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Description: A polyclonal antibody against Arachin Ahy-3. Recognizes Arachin Ahy-3 from Peanut. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Arachin Ahy-3 Polyclonal Antibody, FITC Conjugated |
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A56272 | EpiGentek |
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