ExpressMax Basal Salts | 0145

Athena off ers 3 versions of the basic nutrient-wealthy LB Broth media (Miller, Lennox, and Luria) in addition to their APF Certified equivalents made the use of Athena’s proprietary mixed plant protein hydrolysate: Atholate. The formulations every offer an abundance of peptides and peptones, nutrients and hint elements, and diff er only in sodium chloride content material and whether LB Broth & Animal-Product-Free LB Broth they have been made with casein hydrolysate or Atholate. The differences permit the researcher to choose which system will offer the best osmotic situations for their specific bacterial stress and desired lifestyle situations. Each LB Broth and APF LB Broth may be custom ordered in bulk.

Turbo Broth & Turbo Prime Broth

• Turbo Broth, Turbo Prime Broth and the brand new Turbo Prime-olate  are proprietary media formulations used for the cultivation of recombinant traces of E. coli.
• Both Turbo Prime and Turbo Prime-olate are APF Certified. Turbo Prime-olate is made with Atholate, Athena’s proprietary mixed plant protein hydrolysate.
• The media improve the yield of recombinant proteins and generally produce mobileular yields four to five instances better than that of LB Broth in shake fl ask cultures relying at the stress.
• They have better plasmid replica numbers than LB, that could decorate expression. Each medium makes use of glycerol for a carbon supply and has a wealthy nutrient base of amino acids, nutrients, inorganic minerals and hint minerals.
• The media are buffered at pH 7.2 ± 0.2 with potassium phosphate to save you a extrade in pH and offer a supply of phosphate. These media are perfect because the base for fermentation cultures.

Superior Broth & Superior Prime Broth

Superior Broth, Superior Prime Broth and new Superior Prime-olate, are proprietary media formulations advanced through AthenaES for advanced expression of recombinant proteins in E. coli. The formulations are complex, offering a nitrogen supply, nutrients, and moderate glucose tiers. The media are buff ered at pH 7.2 ± 0.2 to save you acidifi cation of the medium for the duration of metabolism of the glucose. Expression of recombinant proteins need to be triggered at mobileular densities twice that of LB Broth.

Cell yields are generally approximately three to four instances that of LB Broth. Superior Broth indicates the very best plasmid replica numbers many of the AthenaES own circle of relatives of media. This medium is an high-quality preference for large-scale plasmid preparations. Biomass yields will depend at the manner and the stress employed Power Broth & Power Prime Broth Power Broth, Power Prime Broth, and Power Prime-olate are proprietary media formulations advanced through AthenaES for advanced expression of recombinant proteins in E. coli. These media are composed of a complex, wealthy aggregate of amino acids, nutrients, and a carbon supply at better tiers than Terrifi c Broth. The media are buff ered at pH 6.8 ± 0.2.

Power Prime and Power Prime-olate are APF Certified. For expression of recombinant proteins, induction need to be performed at mobileular densities 3 instances that of LB Broth. Cell yields are generally three to four instances that of LB Broth in shake fl ask cultures relying at the traits of the stress. Several reviews recommend that Power Broth and Power Prime Broth can growth the quantity of soluble protein accrued for in any other case insoluble proteins. This medium is a favorable base for fermentation cultures. Biomass yields will rely on the manner and the stress employed.

Hyper Broth

Hyper Broth is an APF Certified proprietary media system advanced through AthenaES for advanced expression of recombinant proteins in E. coli. The medium is a complex, wealthy system which supplies amino acids, nutrients, and glucose at high tiers. This medium yields the very best level of biomass of all AthenaES media. The medium is buff ered at pH 7.three ± 0.2 and has the very best buff ering ability of Athena’s Expression Media, which allows high glucose tiers.

For expression of recombinant proteins, induction need to be performed at mobileular densities 3 instances that of LB Broth. Cell yields are generally five instances that of LB Broth relying at the stress. Plasmid replica numbers are signifi cantly better than they may be for LB Broth. This medium is appropriate because the base for fermentation cultures and is high-quality for feed-batch applications. Biomass yields will rely on the manner and the stress employed. Hyper Broth™ powder is provided with a separate Glucose Nutrient Mix.