Sensitive and precise. Detection limit of 0.05 μM and linearity to 10 μM NAD + / NADH in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent and reading the optical density at time zero and 15 min at room temperature.

High performance. It can be easily automated as a high throughput 96-well plate assay for thousands of samples per day.

  • Method: OD565nm
  • Samples: Cell or tissue extracts
  • Species: All
  • Size: 100 tests
  • Detection limit: 0.05 μM
  • Duration: 6 months

More details

Pyridine nucleotides play an important role in metabolism and therefore there is a continuing interest in monitoring their concentration levels. The quantitative determination of NAD + / NADH has applications in research related to energy transformation and the redox state of cells or tissues. Simple, straightforward, and automation-ready procedures for measuring NAD + / NADH concentration are highly desirable.

The EnzyChrom ™ NAD + / NADH Assay Kit from BioAssay Systems is based on a cyclic lactate dehydrogenase reaction, in which the formed NADH reduces a formazan reagent (MTT). The intensity of the color of the reduced product, measured at 565 nm, is proportional to the concentration of NAD + / NADH in the sample. This assay is highly specific for NAD + / NADH and with minimal interference (<1%) by NADP + / NADPH. Our assay is a convenient method to measure NAD, NADH, and their ratio.

Request

For the sensitive determination of NAD and NADH and the evaluation of the effects of drugs on the metabolism of NAD / NADH.

Leave a comment

Your email address will not be published.