Sensitive and precise. Detection limit of 0.05 μM and linearity to 10 μM NAD + / NADH in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent and reading the optical density at time zero and 15 min at room temperature.
High performance. It can be easily automated as a high throughput 96-well plate assay for thousands of samples per day.
- Method: OD565nm
- Samples: Cell or tissue extracts
- Species: All
- Size: 100 tests
- Detection limit: 0.05 μM
- Duration: 6 months
Pyridine nucleotides play an important role in metabolism and therefore there is a continuing interest in monitoring their concentration levels. The quantitative determination of NAD + / NADH has applications in research related to energy transformation and the redox state of cells or tissues. Simple, straightforward, and automation-ready procedures for measuring NAD + / NADH concentration are highly desirable.
The EnzyChrom ™ NAD + / NADH Assay Kit from BioAssay Systems is based on a cyclic lactate dehydrogenase reaction, in which the formed NADH reduces a formazan reagent (MTT). The intensity of the color of the reduced product, measured at 565 nm, is proportional to the concentration of NAD + / NADH in the sample. This assay is highly specific for NAD + / NADH and with minimal interference (<1%) by NADP + / NADPH. Our assay is a convenient method to measure NAD, NADH, and their ratio.
For the sensitive determination of NAD and NADH and the evaluation of the effects of drugs on the metabolism of NAD / NADH.